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© 1993 SAGE Publications The High-Molecular-Weight Human Mucin Is the Primary Salivary Carrier of ABH, Lea, and Leb Blood Group AntigensDivision of Oral Biology, School of Dentistry, School of Medicine, Box 0512 HSW 604, University of California, San Francisco, CA 94143, Department of Pharmaceutical Chemistry, School of Pharmacy, School of Medicine, Box 0512 HSW 604, University of California, San Francisco, CA 94143, Departments of Psychiatry, Anatomy, and Obstetrics, Gynecology, and Reproductive Sciences, School of Medicine, Box 0512 HSW 604, University of California, San Francisco, CA 94143
Division of Oral Biology, School of Dentistry, School of Medicine, Box 0512 HSW 604, University of California, San Francisco, CA 94143, Department of Pharmaceutical Chemistry, School of Pharmacy, School of Medicine, Box 0512 HSW 604, University of California, San Francisco, CA 94143, Departments of Psychiatry, Anatomy, and Obstetrics, Gynecology, and Reproductive Sciences, School of Medicine, Box 0512 HSW 604, University of California, San Francisco, CA 94143
Division of Oral Biology, School of Dentistry, School of Medicine, Box 0512 HSW 604, University of California, San Francisco, CA 94143, Department of Pharmaceutical Chemistry, School of Pharmacy, School of Medicine, Box 0512 HSW 604, University of California, San Francisco, CA 94143, Departments of Psychiatry, Anatomy, and Obstetrics, Gynecology, and Reproductive Sciences, School of Medicine, Box 0512 HSW 604, University of California, San Francisco, CA 94143 Because many bacteria interact with the carbohydrate portions of receptor molecules, factors controlling glycosylation probably influence the ability of salivary components to mediate bacterial adherence/clearance. Important sources of diversity in glycosylation are the ABO, secretor, and Lewis genes, which code for glycosyltransferases that add specific sugar sequences to the termini of carbohydrate chains of glycolipids and glycoproteins. We identified, by Western blotting, salivary glycoproteins carrying the ABH and Le a or Leb antigens. Samples of whole, unstimulated saliva were obtained from 19 subjects whose blood group was determined by agglutination of red blood cells with specific antisera. After centrifugation, the samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto nitrocellulose. Glycoproteins carrying blood group antigens were identified by staining the blot with monoclonal antisera specific for the A, B, H, Lea, or Leb antigens. The most intensely staining component from all the samples migrated at the same position as the high-molecular-weight mucin. Saliva samples from the nonsecretors contained only the Lea antigen. Samples from the secretors contained one or more of the ABH antigens and, variably, the Leb antigen. In all cases, the salivary blood group antigens corresponded to those found on the red blood cells of the same subject. The functional consequences of the expression of blood group antigens on the high-molecular-weight mucin are not known, but their presence could modulate the adherence of certain oral microorganisms that interact preferentially with this molecule.
Key Words: saliva • mucin • carbohydrate • blood group antigens • bacterial receptors.
Critical Reviews in Oral Biology & Medicine, Vol. 4, No. 3,
325-333 (1993) This article has been cited by other articles:
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