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Critical Reviews in Oral Biology & Medicine
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4(3):407-414 (1993)     Crit Rev Oral Biol Med
© 1993 SAGE Publications

Identification and Localization of G Proteins in Exocrine Glands

Eileen L. Watson

Departments of Oral Biology and Pharmacology, University of Washington, Seattle, WA 98195

Dennis Di Julio

Departments of Oral Biology and Pharmacology, University of Washington, Seattle, WA 98195

Dolphine Oda

Departments of Oral Biology and Pharmacology, University of Washington, Seattle, WA 98195

Kenneth T. Izutsu

Departments of Oral Biology and Pharmacology, University of Washington, Seattle, WA 98195

Constance Oliver

Departments of Microbiology and Immunology, National Institute of Dental Research, National Institute of Health, Bethesda, MD 20892

GTP-binding proteins were identified in rat parotid acinar plasma-enriched membranes (PM) by immunoblot analysis and localized immunohistochemically in the parotid gland as well as in other exocrine glands by using affinity-purified antisera specific for alpha subunits of the G proteins. Isolated rat parotid acinar PM immunoreacted strongly to antisera directed against Gsa, Gi{alpha}1/{alpha}2, Gia3, and Goa; the signal for Goa, however, was weak with crude Go antisera. Immunohistochemical studies to identify and localize Go in rat parotid tissue revealed that antisera to Go{alpha} immunoreacted with ductal cells. In addition, strong immunoreactivity to Goa antisera was noted in ductal cells of other salivary glands including rat submandibular, mouse parotid, and mouse submandibular glands. Light labeling of rat parotid and submandibular gland acinar cells was also noted. In contrast, in the rat and mouse pancreas, Go antisera immunoreacted primarily with islet cells. Ductal cells were negative, but there was light labeling of rat pancreatic acinar cells. The apparent ductal specificity of Goa staining was further verified by demonstrating that Goa antisera immunoreacted strongly with HSG-PA cells, a human transformed salivary ductal cell line. The results demonstrate that rat parotid acinar plasma membranes express a number of G proteins including Go and that Go appears to be selectively expressed in the ductal cells of rat parotid gland and other salivary glands. The selective enrichment of Go in ductal cells suggests that this G protein may play an important role in ductal cell physiology.

Key Words: GTP-binding proteins • Go • salivary glands • ductal cells • immunohistochemistry • immunoblot analysis.

Critical Reviews in Oral Biology & Medicine, Vol. 4, No. 3, 407-414 (1993)
DOI: 10.1177/10454411930040032101
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