Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Critical Reviews in Oral Biology & Medicine
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Archibald, D. W.
Right arrow Articles by Lewis, G. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
4(3):475-478 (1993)     Crit Rev Oral Biol Med
© 1993 SAGE Publications

Effects of Human Salivas on Recombinant HIV-1 Proteins

David W. Archibald

Department of Oral Pathology, University of Maryland Dental School, Department of Microbiology and Immunology, University of Maryland Medical School, 666 West Baltimore Street Baltimore, MD 21201

Carla A. Hebert

Department of Oral Pathology, University of Maryland Dental School, Department of Microbiology and Immunology, University of Maryland Medical School, 666 West Baltimore Street Baltimore, MD 21201

Kevin L. Gregory

Department of Oral Pathology, University of Maryland Dental School, Department of Microbiology and Immunology, University of Maryland Medical School, 666 West Baltimore Street Baltimore, MD 21201

George K. Lewis

Department of Oral Pathology, University of Maryland Dental School, Department of Microbiology and Immunology, University of Maryland Medical School, 666 West Baltimore Street Baltimore, MD 21201

Human saliva appears to contain factors that are inhibitory to HIV-1 infectivity in vitro. We investigated the effect of incubating human whole, parotid, labial minor salivary gland and sublingual/submandibular salivas with recombinant HIV-1 envelope protein (gpl60). Saliva/gpl60 mixtures were run on polyacrylamide gels, transferred to nitrocellulose, and assayed for the presence of gp 160 using monoclonal antibodies or HIV-1-positive sera. Incubation of the gp 160 with whole saliva reduced the intensity of gp 160 bands to 35% of control values. Minor salivary gland saliva reduced the band intensities to 65% of control values, while other saliva types diminished gp160 to 75% of control values. Protease inhibitors had no effect. Components of untreated whole human saliva prevent the detection of the HIV-1 envelope protein gp 160 by antibodies to gp120 and gp41 in immunoblots. The results suggest that complexes between whole saliva factors and certain domains of gp160 block monoclonal antibody binding or are unable to migrate through polyacrylamide gels.

Key Words: saliva • HIV • envelope protein • GP160.

Critical Reviews in Oral Biology & Medicine, Vol. 4, No. 3, 475-478 (1993)
DOI: 10.1177/10454411930040033101
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?