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Critical Reviews in Oral Biology & Medicine
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GLUCAN-BINDING PROTEINS OF THE ORAL STREPTOCOCCI

J.A. Banas1,* and M.M. Vickerman2,3

1 Center for Immunology and Microbial Disease, Albany Medical College, MC-151, MS-239, 47 New Scotland Avenue, Albany, NY 12208; and
2 Department of Oral Surgery and Hospital Dentistry, School of Dentistry, and Department of Microbiology and Immunology, School of Medicine, Indiana University, Indianapolis, IN;


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  		Figure. (A) Two-day-old sucrose-dependent biofilms produced within 24-well microtiter dishes according to previously published procedures (Banas et al., 2001). The GbpA-strain produced a flatter biofilm that coated the surface more evenly. The GbpC-strain produced a biofilm that was at least twice as thick as that produced by wild-type S. mutans. (B) We estimated the thicknesses of the biofilms formed by wild-type and GbpC-strains by inverting the contrast (making the background white and cells black) of microtiter well images and using the software program NIH Image for densitometry.

 

Critical Reviews in Oral Biology & Medicine, Vol. 14, No. 2, 89-99 (2003)
DOI: 10.1177/154411130301400203
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