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Critical Reviews in Oral Biology & Medicine
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MAST CELLS AND ORAL INFLAMMATION

Laurence J. Walsh

School of Dentistry, The University of Queensland, 200 Turbot Street, Brisbane, QLD 4000, Australia; l.walsh{at}uq.edu.au


Figure 1
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  		Figure 1. Characteristic morphology and perivascular location of a mast cell (arrow), as seen in a one-micrometer-thick resin section stained with toluidine blue. The mast cell is close to the abluminal surface of the endothelium of this post-capillary venule. The basal epithelial layer (E) is seen in the upper right. Original magnification X1000. Scale bar indicates 5 µm.

 

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  		Figure 2. Triple-immunoperoxidase-labeled frozen section showing the relationship among mast cells, blood vessels, and nerves. Peripheral nerves stained with antibodies to neurofilament protein are brown, while the CD31 (PECAM-1) adhesion molecule on the luminal surface of the blood vessel is stained pink. Tryptase in mast cells is stained black. Several mast cells are present in the section. Two of these (indicated by arrows) are juxtaposed between nerves and blood vessels. Original magnification X400. Scale bar represents 20 µm.

 

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  		Figure 3. Stained cytocentrifuge preparations of purified mast cells, showing metachromatic staining of the granules with toluidine blue (panel A). Immunohistochemical staining reveals the presence of both chymase (panel B) and TNF (panel C) in the mast cell granules. Original magnification X1000. Scale bar indicates 5 µm.

 

Critical Reviews in Oral Biology & Medicine, Vol. 14, No. 3, 188-198 (2003)
DOI: 10.1177/154411130301400304
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